Mercodia inc 10112801 a high quality enzyme immunoassay for the quantification of insulin in serum or plasma. The human insulin elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of human insulin and proinsulin in serum, plasma, cell culture supernatants and urine. In the same year, 1st international standard of bovine insulin, the potency of which is 25. Plasma free insulin isoinsulin elisa enzymelinked immunosorbent assay. No crossreactivity to cpeptide or proinsulin des 32 or split 3233 insignificant or no crossreactivity to insulin. Insulin secretion was evaluated in glucosestimulated insulin secretion test using static incubation of mnsib2mlabeled or mnsiscrlabeled islets at low 1. Islet viability assay and glucose stimulated insulin.
The inhouse elisa would need to be able to detect insulin concentrations in that range in order for it to be useful to our group. Human insulin elisa kit for serum, plasma, cell culture. Immunogen recombinant human insulin application for research use only. Summary of protocol sheet mercodia ovine insulin elisa. The alpco insulin elisa is designed for the quantitative determination of insulin in serum and plasma. A protocol to enhance ins1e and min6 functionalitythe use.
Mercodia inc isoinsulin elisa kit fisher scientific. Mercodia glucagon elisa is an assay intended to measure the pancreatic hormone glucagon in plasma and serum. Mercodia isoinsulin elisa directions for use 10112801 reagents for 96 determinations manufactured by mercodia ab, sylveniusgatan 8a, se754 50 uppsala, sweden gebrauchanweisung in deutsch finden sie under folgenden link. Exercise counteracts the effects of shortterm overfeeding and reduced physical activity independent of energy. Spontaneous presentation of insulin is essential for initiating autoimmune type 1 diabetes in nonobese diabetic mice1,2. Cells were treated with glucose to detect insulin secretion and cells were lysed to detect the insulin content. Principle of the assay the alpco insulin elisa is a sandwich type immunoassay. An elisa that measured the concentration of insulin directly, such. Intended use this rat mouse insulin elisa kit is used for the nonradioactive quantification of insulin in mouse and rat sera. The quantikine humancanineporcine insulin immunoassay is a 4. Mercodia insulin elisa is a solid phase twosite enzyme immunoassay. Insulin in the sample reacts with anti insulin antibodies bound to microtitration wells and. Summary of protocol sheet not provided for full details see under test procedure. The kit is for research and manufacturing use only and is not approved for diagnostic use in humans or animals the lod for this.
Mercodia was founded in 1991 and develops, manufactures, and markets diagnostic kits based on elisa technology, for clinical and research applications. Mercodia insulin elisa provides a method for the quantitative determination of human insulin in serum or plasma. Mercodia ratmouse insulin fia provides a method for the quantitative. Increased proinsulin over insulin ratio may be found in rat and mouse models of hyperglycemia 4,5, or after manipulation of proinsulinprocessing enzymes 6,7. Summary and explanation of the test insulin is the. Mercodia high range rat insulin elisa provides a method for the quantitative determination of insulin in rat. The mercodia insulin assays have little or no crossreactivity to insulin.
Evaluation of commercially available assays for the. Competitive elisa insulin assay protocol materials. Humancanineporcine insulin quantikine elisa kit dins00. With ivcce labelled elisas for the most prominent biomarkers within metabolic related research, weve supported all levels of studies for. The content of this kit and their residues must not be allowed to come into contact with ruminating. The porcinespecific insulin assay 1 is a solid phase twoside sandwich elisa optimized for the quantification of equine insulin in serum or plasma.
Insulin elisa specific measurement of insulin mercodia. Mercodia iso insulin elisa is a solid phase twosite enzyme immunoassay. After 48 h insulin secretion upon glucose stimulation with the addition of theophylline 10 mm in both low 1. Exercise training caused a significant decrease in the. It is based on the sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants. Insulin control high, low mercodia diabetesantigen control rat, mouse l, m, h 10122001 reagent kit for 1 x 96 kit each mercodia ultrasensitive rat insulin elisa kit 10125101 contains reagents for 96 wells, sufficient for 42 samples and one calibrator standard curve in duplicate. Low sample volume protocols requiring as little as 5 l.
Restoration of glucosestimulated cdc42pak1 activation. Insulin elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Following the experiment period, blood samples were taken from all animals and analyzed for experimental indexes via sandwich elisa. Mercodia isoinsulin elisa provides a method for the quantitative determination of insulin in human serum or plasma. The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. Catalog number 101110 10 x 96 determinations or 10111 96 determinations. Mercodia ab, uppsala, sweden and blood glucose concentrations were measured from samples collected at.
Each mercodia ultrasensitive rat insulin elisa kit 10125101. Laboratory procedure manual centers for disease control. Principle of the method the human insulin solidphase sandwich elisa enzymelinked immunosorbent assay is designed to measure the amount of the target bound between a matched antibody pair. Background insulin is the principal hormone responsible for the control of glucose metabolism. As with all diagnostic tests, a definitve clinical diagnosis should not be based on the results of. Our elisa assay kits are complete, fully validated and readytouse for a. Mercodia isoinsulin elisa directions for use 10112801 reagents for 96 determinations manufactured by mercodia ab, sylveniusgatan 8a, se754 50 uppsala, sweden gebrauchanweisung in. Summary and explanation of the test insulin is the principal hormone responsible for the control of glucose metabolism. We used the kit with insulin secreting human cell lines following the manufacturers protocol. As with all diagnostic tests, a definitve clinical diagnosis should not be based on the results of a single test, but should be made by the physician after all clinical findings have been evaluated. Endurance exercise training restores diabetesinduced. Background insulin is the principal hormone responsible for the control of glucose. For mercodia insulin elisa, an alternative sequence protocol see technical note 340143 may be used that do not detect insulin glargine or glargine metabolites m1 or m2. Glucagon elisa, product flyer bioconnect diagnostics.
Insulin elisa kit provides a method for the quantitative determination of human insulin in serum or plasma. Accurate and reliable results are critical when taking a scientific concept from benchtobedside. Principle of the method the human insulin solidphase sandwich elisa enzymelinked immunosorbent assay. Exercise counteracts the effects of shortterm overfeeding and reduced physical activity independent of energy imbalance in healthy young men. A major set of pathogenic cd4 t cells specifically recognizes the 1220 segment of the insulin bchain b. Summary and explanation of the test insulin is the principal hormone responsible for. Macronutrients, fatty acids, adiponectin, insulin, and igf. Summary and explanation of the test insulin is the principal hormone. Calibrated against 1st international reference preparation 66304 for human insulin. The assay will recognize both the natural and recombinant insulin.
The human insulin elisa kit quantifies human insulin in serum. Each mercodia ultrasensitive rat insulin elisa kit 10 125101. Test principle mercodia glucagon elisa is a solid phase twosite enzyme immunoassay based on the sandwich technique, in which two monoclonal. The university of minnesota periodically refines these laboratory. Mercodia canine insulin elisa is a solid phase twosite enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants. Plasma samples may also be used but application to samples of other biological fluids may need validation by the user. We provide highquality elisa kits to facilitate your research advancements. A high quality enzyme immunoassay for the quantification of insulin in serum or plasma.
Ultra sensitive mouse insulin elisa kit crystal chem inc. What is the most cost effective insulin elisa kit available for purchase. Before these standards, in 1974, 1st international reference preparation of human insulin for immunoassay was provided as 3 iuampoule. During incubation insulin in the sample react with. Le istruzioni per luso sono reperibili in italiano all. Insulin was measured with the mercodia insulin elisa kit 10111 mercodia, uppsala, sweden from 25 l of undiluted, skimmed human milk, according to the protocol of the manufacturer. We used the kit with insulinsecreting human cell lines following the manufacturers protocol. The mouse insulin elisa ultra sensitive is used to quantify levels of mouse insulin in serum, plasma, cell culture, and fluid using only a 5 l sample volume. The human insulin elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of human insulin and proinsulin in serum. This kit is for quantitating insulin from bovine, human, or porcine sources. Diagenics limited, south house 3, bond avenue, bletchley, milton keynes, mk1 1sw registered in england no. Filter paper blood spot enzyme linked immunoassay for insulin. The mercodia mouse insulin elisa is an easytouse assay based on highly. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments.
The insulin human elisa kit is intended for the quantitative measurement insulin in human serum or plasma. Insulin present in samples or standards binds to the anti insulin antibodies preadsorbed on the microtiter plate. Enzymelinked immunosorbent assay elisa is a popular analysis tool in diverse research areas such as immunology, neuroscience and cancer. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. The kit worked perfectly when detecting high content of insulin. Address mercodia ab, sylveniusgatan 8a, se754 50 uppsala, sweden version 3. The human insulin elisa kit ab200011 is easy to work with and reproducible. Mercodia insulin elisa provides a method for the quantitative determination of human insulin in serum or. Im looking to purchase an insulin elisa kit, and hopefully for a good price. Can be combined with the highly specific mercodia human insulin elisa to enable the calculation of insulin analog concentrations by subtracting endogenous insulin. To order mercodia products, please contact us discounts are available for orders of larger quantities. Mercodia proinsulin elisa is a solid phase twosite enzyme immunoassay. Glucagon measurements are used in the diagnosis and treatment of patients with various. The 96well microplate is coated with a monoclonal antibody specific for insulin.
Insulin secretion is triggered by elevated glucose concentration in the. Insulin control high, low mercodia diabetesantigen control rat, mouse l, m, h 10122001 reagent kit for 1 x 96 kit each mercodia ultrasensitive rat insulin elisa kit 10125101 contains reagents for. The kit is for research and manufacturing use only and is not approved for diagnostic use in humans or animals the lod for this kit is 125 pgml. No crossreactivity to cpeptide or proinsulin des 32 or split 3233 insignificant or no crossreactivity to insulin analogs. Intended use mercodia ovine insulin elisa provides a method for the quantitative determination of insulin in ovine serum and plasma. Mercodia insulin elisa 10 pack walhin jp, richardson jd, betts ja, thompson d. Elabscience offers over thousands of highly sensitive and specific elisa assay kits that can solve your immunoassay problems. Insulin concentrations in supernatants were measured using a human insulin elisa kit mercodia, uppsala, sweden.